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recD2 [2019-08-08 09:18:55]
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recD2 [2019-08-08 09:18:55]

5'-3' DNA helicase replication fork progression
locus
BSU_27480
pI
5.08
mw
88.99 kDa
protein length
798 aa Sequence Blast
gene length
2397 bp Sequence Blast
function
replication fork progression
product
5'-3' DNA helicase
essential
no
ec
3.6.4.12
synonyms
yrrC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]

    • Gene

    Coordinates
    2,806,286 2,808,682

    Phenotypes of a mutant

  • modest increase in spontaneous mutation rate [Pubmed|24443534]
  • [gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2] [gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG] as well as [gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2] [gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|search|ruvB ]combined mutants are not viable [pubmed|28527403]

    • The protein

    Protein family

  • RecD family (single member, according to UniProt)

    • Structure

  • [PDB|3E1S] (from Deinococcus radiodurans, 28% identity, from aa 178 - 767) [pubmed|18668125]

    • Expression and Regulation

    Operons

    genes
    [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A858 (yrrC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/858 NBRP B. subtilis, Japan]
  • BKE27480 ([gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGCTGTCCCTCCTCCT, downstream forward: _UP4_TAAAATGCTCCCGTGCAAGC
  • BKK27480 ([gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGCTGTCCCTCCTCCT, downstream forward: _UP4_TAAAATGCTCCCGTGCAAGC

    • References

  • 21170359,24443534,28527403,18668125